Flow Injection Analysis with Luminol Chemiluminescence Detection for Evaluation of Quenching Effects of Grape Seed Extracts and Polyphenolics against Reactive Oxygen Species

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  • WADA Mitsuhiro
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • KATOH Masayuki
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • KIDO Hirotsugu
    Mitsubishi Chemical Corporation, Specialty Chemicals Company
  • NAKASHIMA Mihoko N.
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • KURODA Naotaka
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University
  • NAKASHIMA Kenichiro
    Graduate School of Biomedical Sciences, Course of Pharmaceutical Sciences, Nagasaki University

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Other Title
  • フローインジェクション‐ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価
  • フローインジェクション-ルミノール化学発光検出によるブドウ種子抽出物及びポリフェノール類の活性酸素種消去能評価
  • フローインジェクション ルミノール カガク ハッコウ ケンシュツ ニ ヨル ブドウ シュシ チュウシュツブツ オヨビ ポリフェノールルイ ノ カッセイ サンソシュ ショウキョノウ ヒョウカ

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Description

A simple and rapid FIA method for measurement of quenching effects of grape seed extracts and polyphenolics against reactive oxygen species (ROS) was developed. This method is based on the determination of luminol chemiluminescence generated with ROS such as hydroxyl radical (•OH), singlet oxygen (1O2), peroxynitrite (ONOO−) and superoxide anion (O2−). More than 1 sample/min could be measured with satisfactory precision. The total reagent amount in this method could be reduced to one tenth of that for our previous batch method. The grape seed extracts A and B were successfully evaluated by the proposed method. The EC50 of cyanidin as a representative polyphenolic against •OH, 1O2, ONOO• and O2− were 50±4, 148±8, 36±4, and 95±8 ng/assay (n=3), respectively. Except for chalcone, the other polyphenolics such as pelargonidin, delphinidin and trans-resveratrol also showed the high quenching effects against ROS.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 55 (12), 931-936, 2006

    The Japan Society for Analytical Chemistry

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