Rapid Determination of Cadmium in Rice by Immunochromatography Using Anti-(Cd-EDTA) Antibody Labeled with Gold Particle

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Other Title
  • 金コロイド標識抗カドミウム‐エチレンジアミン四酢酸錯体モノクローナル抗体を用いるイムノクロマトグラフィーによる米中カドミウムの簡易定量
  • 金コロイド標識抗カドミウム-エチレンジアミン四酢酸錯体モノクローナル抗体を用いるイムノクロマトグラフィーによる米中カドミウムの簡易定量
  • キン コロイド ヒョウシキ コウカドミウム エチレンジアミン 4サクサン サクタイ モノクローナル コウタイ オ モチイル イムノ クロマトグラフィー ニ ヨル ベイチュウ カドミウム ノ カンイ テイリョウ

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Description

A simple and rapid immunochromatography (IC) system for the detection of Cd in rice has been developed. The produced anti-Cd-EDTA antibody exhibited the equilibrium dissociation constant (Kd) for Cd-EDTA at 1.3 × 10−8 M, which was almost 100-fold tighter than Kd for the other tested EDTA-metal complex. Cadmium in rice was extracted by HCl, and the extract was introduced into a column to remove co-existing metals (such as zinc, manganese and magnesium) while retaining cadmium. After a pretreatment, IC could detect cadmium in the range from 0.01 to 0.1 mg L−1. Fifty brown rice samples were tested with the column treatment and the IC assay. The estimated cadmium concentrations from the assay were evaluated by a comparison with the results of a nitrate treatment and ICP-AES analysis for the samples. Two measurements were highly correlated, with a correlation coefficient of 0.94.<br>

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 56 (1), 29-36, 2007

    The Japan Society for Analytical Chemistry

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