ISFET-Type L-Glutamate Sensor Using Thermophilic Glutamine Synthetase from a Thermophile

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  • IIDA Takeaki
    Department of Applied Chemistry, Faculty of Engineering, Saitama University
  • KAWABE Takeshi
    Department of Applied Chemistry, Faculty of Engineering, Saitama University
  • NOGUCHI Fumio
    Department of Applied Chemistry, Faculty of Engineering, Saitama University
  • MITAMURA Takashi
    Department of Applied Chemistry, Faculty of Engineering, Saitama University
  • NAGATA Kazuhiko
    Research and Development Center, Unitika Ltd
  • TOMITA Kosuke
    Research and Development Center, Unitika Ltd

Bibliographic Information

Other Title
  • 好熱菌の耐熱性酵素グルタミンシンテターゼを用いるISFET型L-グルタミン酸センサー

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Description

An ion sensitive field effect transistor (ISFET) type L-glutamate sensor was studied using an immobilized thermophilic enzyme glutamine synthetase from a moderate thermophilic bacterium. The ISFET glutamate sensor, on which the glutamine synthetase and albumin were immobilized by glutaraldehyde, was able to determine the L-glutamate concentration in the range of 0.5-400 mmol.dm-3. The response time of the glutamate sensor was about 3 min. The selectivity of the glutamate sensor was studied by measuring its responses to various amino acids, and. the sensor did not respond to any other amino acids except D-glutamate and α-methylglutamate. Therefore, the glutamate sensor was found to have high selectivity for L-glutamate. The sensor also revealed high reproducibility. The coefficient of variation of the sensor was determined to be 9.3% for 10 samples containing 10 mmol.dm-3 L-glutamate. The initial output voltage of the glutamate sensor was retained for 15 d. However, the output voltage decreased to about 45% of the initial output voltage after 30 d.

Journal

  • NIPPON KAGAKU KAISHI

    NIPPON KAGAKU KAISHI 1987 (10), 1817-1821, 1987-10-10

    The Chemical Society of Japan

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