Conformational Transition of Copolypeptides with both Hydrophilic and Hydrophobic Groups Side Chains

DOI Web Site
  • UCHIDA Chieko
    Department of Biological Science and Technology, Faculty of Engineering, Gun-ma University
  • IIZUKA Yasuko
    Yabuzuka Medical Center
  • MURAOKA Ruriko
    Department of Biological Science and Technology, Faculty of Engineering, Gun-ma University
  • WAKAMATSU Kaori
    Department of Biological Science and Technology, Faculty of Engineering, Gun-ma University
  • OYA Masanao
    Department of Biological Science and Technology, Faculty of Engineering, Gun-ma University

Bibliographic Information

Other Title
  • 親水性基,疎水性基を側鎖に持つアミノ酸共重合体の各種緩衝液中での二次構造変化

Description

Copolypeptides with both hydrophilic and hydrophobic side chains, i. e. Poly (Lys, Ala), Poly (Lys, Abu), Poly (Lys, Ser) and Poly (Asp, Ala) were synthesized. Acid-, base-, salt-, and pH-dependent conformational transition of these copolypeptides were compared with those of Poly (Lys) at various buffer solutions by using circular dichroism spectroscopy. Poly (Lys, Ala) was in the helix conformat ion in a citric acid-NaOH at pH 4.53, while random-coil was mainly observed in a KC1 solution at pH 6.25. Poly (Lys, Ala) was also in the helix in the phosphate buffer solution at pH 6.86, but Poly (Lys) was in the coiled form. Since Poly (Lys, Ser) rarely took the helix conformation, intermolecular hydrogen bonding between hydroxyl groups of the Poly (Lys, Ser)may be involved. Confo rmational change of these copolypeptides is dependent on both pH and coexisting ions.

Journal

  • NIPPON KAGAKU KAISHI

    NIPPON KAGAKU KAISHI 1995 (5), 382-387, 1995-05-10

    The Chemical Society of Japan

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