書誌事項
- タイトル別名
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- Cadystins((.GAMMA.-EC)nG) Synthesis Ability of Carboxypeptidase(CPase).
- Cadystins[(γ-EC)nG]Synthesis Ability of Carboxypeptidase(CPase)
- Cadystins ガンマーEC nG Synthesis Ability of Carboxypeptidase CPase
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The authors characterized a enzyme that is responsible for (γ-EC)nG synthesis in rice root and it was confirmed that at least one of the (γ-EC)nG synthases in the rice root may be carboxypeptidase (CPase). We examined the ability of CPases in vitro to (γ-EC)nG synthesis and to confirm that CPase in rice plant have the ability to synthesis (γ-EC)nG. The structural gene of the rice CPase was ligated to the expression vector, transformed into E. coli and expressed. All of the CPase (CPase W, Y, and A) were examined synthesized having ability to (γ-EC)2G in vitro. On the contrary, none of the aminopeptidase synthesize it examined in this study. The specific inhibitor of CPase inhibited (γ-EC)2G synthesis. The optimum pHs of the synthesis with each CPase are the same as optimum pHs for the hydrolytic activity of the respective enzymes. The (γ-EC)nG synthesis activity was increased through CPase gene transformation E. coli. These results suggest that at least one of the (γ-EC)nG synthases is CPase.
収録刊行物
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- 環境科学会誌
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環境科学会誌 12 (4), 393-398, 1999
社団法人 環境科学会
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詳細情報 詳細情報について
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- CRID
- 1390282679398026880
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- NII論文ID
- 10005338757
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- NII書誌ID
- AN10165252
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- ISSN
- 18845029
- 09150048
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- NDL書誌ID
- 4925313
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可