39.Click chemistryを利用したイソレスペデジン酸カリウム標的タンパク質の精製(口頭発表)

DOI

書誌事項

タイトル別名
  • 39. Purification of the Target Protein for Potassium Isolespedezate Using Click Tagging Technology

抄録

Most leguminous plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythm. Potassium isolespedezate 1 is a leaf-opening factor of leguminous plant, Cassia obtusifolia. We planed to purify the target protein of 1, by labeling with FLAG peptide-tag. First, we labeled the target protein with azide group by incubating the iodoacetamide-type probe (2) with the motor cell protoplasts, which is the target cell of 1, and then, succeeded in introduction of FLAG using click chemistry. Then, the 83kDa cytoplasmic protein (CTPL; cytosolic target protein of lespedezate 1) was detected and purified as a target protein of 1. The analysis of CTPL by using LC-MS/MS revealed that CTPL shares homology with MetE (5-methyl-tetrahydropteroyltrigultamate-homocysteine methyltransferase).

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282679399766912
  • NII論文ID
    110007468299
  • DOI
    10.18978/jscrpanb.44.0_53
  • ISSN
    21896313
    09191887
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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