39. Purification of the Target Protein for Potassium Isolespedezate Using Click Tagging Technology
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- Manabe Yoshiyuki
- Grad. School of Science, Tohoku Univ.
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- Mukai Makoto
- Grad. School of Science, Tohoku Univ.
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- Ueda Minoru
- Grad. School of Science, Tohoku Univ.
Bibliographic Information
- Other Title
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- 39.Click chemistryを利用したイソレスペデジン酸カリウム標的タンパク質の精製(口頭発表)
Abstract
Most leguminous plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythm. Potassium isolespedezate 1 is a leaf-opening factor of leguminous plant, Cassia obtusifolia. We planed to purify the target protein of 1, by labeling with FLAG peptide-tag. First, we labeled the target protein with azide group by incubating the iodoacetamide-type probe (2) with the motor cell protoplasts, which is the target cell of 1, and then, succeeded in introduction of FLAG using click chemistry. Then, the 83kDa cytoplasmic protein (CTPL; cytosolic target protein of lespedezate 1) was detected and purified as a target protein of 1. The analysis of CTPL by using LC-MS/MS revealed that CTPL shares homology with MetE (5-methyl-tetrahydropteroyltrigultamate-homocysteine methyltransferase).
Journal
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- The Janapese Society for Chemical Regulation of Plants, Abstract
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The Janapese Society for Chemical Regulation of Plants, Abstract 44 (0), 53-, 2009
The Janapese Society for Chemical Regulation of Plants
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Details 詳細情報について
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- CRID
- 1390282679399766912
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- NII Article ID
- 110007468299
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- ISSN
- 21896313
- 09191887
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed