39. Purification of the Target Protein for Potassium Isolespedezate Using Click Tagging Technology

DOI

Bibliographic Information

Other Title
  • 39.Click chemistryを利用したイソレスペデジン酸カリウム標的タンパク質の精製(口頭発表)

Abstract

Most leguminous plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythm. Potassium isolespedezate 1 is a leaf-opening factor of leguminous plant, Cassia obtusifolia. We planed to purify the target protein of 1, by labeling with FLAG peptide-tag. First, we labeled the target protein with azide group by incubating the iodoacetamide-type probe (2) with the motor cell protoplasts, which is the target cell of 1, and then, succeeded in introduction of FLAG using click chemistry. Then, the 83kDa cytoplasmic protein (CTPL; cytosolic target protein of lespedezate 1) was detected and purified as a target protein of 1. The analysis of CTPL by using LC-MS/MS revealed that CTPL shares homology with MetE (5-methyl-tetrahydropteroyltrigultamate-homocysteine methyltransferase).

Journal

Details 詳細情報について

  • CRID
    1390282679399766912
  • NII Article ID
    110007468299
  • DOI
    10.18978/jscrpanb.44.0_53
  • ISSN
    21896313
    09191887
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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