39.Click chemistryを利用したイソレスペデジン酸カリウム標的タンパク質の精製(口頭発表)
書誌事項
- タイトル別名
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- 39. Purification of the Target Protein for Potassium Isolespedezate Using Click Tagging Technology
抄録
Most leguminous plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythm. Potassium isolespedezate 1 is a leaf-opening factor of leguminous plant, Cassia obtusifolia. We planed to purify the target protein of 1, by labeling with FLAG peptide-tag. First, we labeled the target protein with azide group by incubating the iodoacetamide-type probe (2) with the motor cell protoplasts, which is the target cell of 1, and then, succeeded in introduction of FLAG using click chemistry. Then, the 83kDa cytoplasmic protein (CTPL; cytosolic target protein of lespedezate 1) was detected and purified as a target protein of 1. The analysis of CTPL by using LC-MS/MS revealed that CTPL shares homology with MetE (5-methyl-tetrahydropteroyltrigultamate-homocysteine methyltransferase).
収録刊行物
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- 植物化学調節学会 研究発表記録集
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植物化学調節学会 研究発表記録集 44 (0), 53-, 2009
一般社団法人 植物化学調節学会
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詳細情報 詳細情報について
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- CRID
- 1390282679399766912
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- NII論文ID
- 110007468299
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- ISSN
- 21896313
- 09191887
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可