Danshen Inhibits Oxysterol-induced Endothelial Cell Apoptosis In Vivo

DOI Web Site PubMed 1 Citations 31 References
  • Nakazawa Toru
    National Cardiovascular Center Research Institute, Osaka, Japan. Tokyo Medical and Dental University Graduate School, Tokyo, Japan. First Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan.
  • Xui Ning
    National Cardiovascular Center Research Institute, Osaka, Japan.
  • Hesong Zeng
    National Cardiovascular Center Research Institute, Osaka, Japan.
  • Kinoshita Mikio
    National Cardiovascular Center Research Institute, Osaka, Japan.
  • Chiba Tsuyoshi
    National Cardiovascular Center Research Institute, Osaka, Japan. Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
  • Kaneko Eiji
    Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
  • Yui Katsumasa
    Tokyo Medical and Dental University Graduate School, Tokyo, Japan.
  • Shimokado Kentaro
    National Cardiovascular Center Research Institute, Osaka, Japan. Tokyo Medical and Dental University Graduate School, Tokyo, Japan.

Search this article

Abstract

Oxysterols induce apoptosis in vascular endothelial cells in vitro, but it is not clear whether they do so in vivo. We intravenously injected an oxysterol, cholestan-3β, 5α, 6β-triol, into rats and quantitatively analyzed endothelial cell apoptosis in the aorta. Oxysterol significantly promoted apoptosis in a time- and dose-dependent fashion. The apoptosis had increased 4.5-fold 6 hrs after injection, and returned to the background level at 48 hrs. An extract of the Chinese herb Danshen as well as probucol abolished triol-induced endothelial cell apoptosis in vitro and in vivo. Since apoptotic cells are quickly cleared, oxysterol-induced apoptosis could significantly affect endothelial integrity over a long period of time. Radical scavengers may be useful for the prevention of endothelial damage.

Journal

Citations (1)*help

See more

References(31)*help

See more

Keywords

Details

Report a problem

Back to top