書誌事項
- タイトル別名
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- Detection of Legionella Species in Environmental Water Samples by Loopamp Legionella Detection Reagents Kit
- カンキョウスイ カラノ Loopamp レジオネラ ケンシュツ シヤク キット オ モチイタ Legionellaゾクキン ノ ケンシュツ
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Detection of Legionella species using the loop-mediated isothermal amplification (LAMP) method was investigated with the “Loopamp Legionella detection reagents kit E” (Eiken Chemical Co., Ltd.). The detection limit of the LAMP method was 3.7×101 CFU/test, approximately equivalent to the limit claimed by the instructions (60 CFU/test). The LAMP method detected positive reactions for 17 of 100 Legionella samples which gave negative reactions by the culture method, indicating that the sensitivity of the LAMP method was higher than the culture method. No false positive results were obtained in the screening of 5 organisms other than Legionella species. All visual results were equal to those obtained using a turbidity meter. The LAMP method can specifically detect Legionella species at levels lower than the detection limit of the conventional culture method in a short time, so is useful for the prevention of nosocomial Legionella infection.
収録刊行物
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- 環境感染
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環境感染 22 (2), 75-78, 2007
日本環境感染学会
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詳細情報 詳細情報について
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- CRID
- 1390282679414653312
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- NII論文ID
- 10024358116
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- NII書誌ID
- AN1019475X
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- ISSN
- 18842429
- 09183337
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- NDL書誌ID
- 8883685
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可