琉球在来豚アグー精子における冷却処理前の室温放置が凍結融解後の精子性状に及ぼす影響

DOI

書誌事項

タイトル別名
  • Standing Periods at Room Temperature before the Onset of Cooling Procedure are Unnecessary to Well Accomplish the Sperm Cryopreservation in Okinawa Native Pig, Agu

抄録

The technical establishment of boar sperm cryopreservation is indispensable for constantly supplying fine spermatozoa to effectively breed rare Okinawa native pig, Agu. Hence, the present study was carried out to determine whether the standing periods of spermatozoa before the onset of cooling procedure had effects on its freezability in association with the resistance to chilling injury, assessed by the integrity of plasmalemma, the acrosomal proteolytic activity, the motile patterns, and the in vitro fertilizing capacity. Ejaculated spermatozoa from four Agu and two Landrace boars were washed by centrifugation within 10 min after collection, suspended in Beltsville thawing solution, and then allowed to stand for 0, 1 or 2 hour at room temperature until dilution with BF5 freezing solution. In Agu, the elongation of standing periods before cooling caused significant (p<0.05) decreases in the integrity of plasmalemma, the proteolytic activity of acrosomal contents, and the rate of motile cells and progressive motility in frozen-thawed spermatozoa. Moreover, the post-thawed spermatozoa without the standing period possessed the highest level of the penetrability to in vitro matured oocytes. However, the influences of the standing periods upon the freezability were not found in Landrace spermatozoa as Agu spermatozoa. Therefore, it is concluded that the freezing method without the standing periods before the onset of cooling procedure is appropriate for cryopreservation of ejaculated spermatozoa in Agu.

収録刊行物

詳細情報 詳細情報について

  • CRID
    1390282679417928448
  • NII論文ID
    130004185290
  • DOI
    10.11461/jwaras1968.50.35
  • ISSN
    18846394
    09143459
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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