Analysis of Lactic Acid Bacterial Flora during Miso Fermentation

  • ONDA Takumi
    Food/Brewing section, Yamanashi Industrial Technology Center
  • YANAGIDA Fujitoshi
    The Institute of Enology and Viticulture, Yamanashi University
  • UCHIMURA Tai
    Department of Applied Biology and Chemistry, Tokyo University of Agriculture
  • TSUJI Masao
    Food/Brewing section, Yamanashi Industrial Technology Center
  • OGINO Satoshi
    Food/Brewing section, Yamanashi Industrial Technology Center
  • SHINOHARA Takashi
    The Institute of Enology and Viticulture, Yamanashi University
  • YOKOTSUKA Koki
    The Institute of Enology and Viticulture, Yamanashi University

Search this article

Description

This article describes a microbiological study of lactic acid bacteria involved in the fermentation process of Miso. The bacteria were counted and isolated from Miso during fermentation and, based on the results of traditional phenotypic tests, divided into nine groups. The isolates were identified by biochemical analysis and 16S rRNA sequence analysis. During Miso fermentation, the halophilic bacterium Tetragenococcus halophilus increased moderately. The non-halophilic strains displayed a complex growth pattern and were identified as Enterococcus faecium, Enterococcus durans, Enterococcus faecalis, Pediococcus acidilactici, Pediococcus pentosaceus, Lactobacillus plantarum and Weissella confusa. The predominant species throughout the fermentation process were T. halophilus, E. faecium and E. durans. Among them, only the strains of E. faecalis and E. durans produced bacteriocins that had an antibacterial effect on B. subtilis, but had none on T. halophilus. The bacteriocin producers appear to play an important role in maintaining normal bacterial flora during Miso fermentation.<br>

Journal

Citations (2)*help

See more

References(44)*help

See more

Details 詳細情報について

  • CRID
    1390282679433304704
  • NII Article ID
    10011999900
  • NII Book ID
    AA11320122
  • DOI
    10.3136/fstr.9.17
  • ISSN
    18813984
    13446606
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

Report a problem

Back to top