デキストラナーゼ精製酵素の諸性質

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  • Some Properties of the Purified Dextranase
  • デキストラナーゼ セイセイ コウソ ノ ショ セイシツ Aspergillus ustus 237 ノ セイサン スル デキストラナーゼ ニ カンスル ケンキュウ 2

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Dextranase which was produced by Aspergillus ustus 237 was purified to approximately 1000 folds by means of ammonium sulfate fractionation, DEAF-cellulose column. chromatography and Bio-Gel P-150 column chromatography.<br> Purified enzyme was appeared to be homogenous in disc-electrophoretic analysis and its molecular weight was estimated to be approximately 35, 000 by gel-filtration on Bio-Gel P-150.<br> Optimum pH and temperature for the enzyme reaction were pH 5.7 and 50°C, respectively. The enzyme was stable in the pH range of 5.5 to 8.5 and also at the temperatures less than 40°C, while more or less 50% of the enzyme activities were inactivated by heating to 50°C for 15 minutes at pH 7.0. However, it was found that the enzyme showed the remarked heat-tolerance in the presence of some cations, such as Cr3+ and Al3+. The dextranase activity was inhibited completely by Hg2+, Ag+ and permanganate and was scarcely by chelating agents reductants.

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