寒天平板による乳酸菌の糖類発酵性新試験法

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  • 岡田 早苗
    東京農業大学総合研究所菌株保存室
  • 内村 泰
    東京農業大学農芸化学科応用微生物学研究室
  • 小原 直弘
    東京農業大学農芸化学科応用微生物学研究室
  • 小崎 道雄
    東京農業大学農芸化学科応用微生物学研究室

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タイトル別名
  • A New Method of Sugar Fermentation Test for Identification of Lactic Acid Bacteria
  • カンテン ヘイバン ニヨル ニュウサンキン ノ トウルイ ハッコウセイ シン

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The sugar fermentation test is an important criteria in identification of lactic acid bacteria. Usually, the sugar fermentation is detected by acidformation from a sugar given in growth media. In the ordinary method, acid-formation is indicated by the value of titration of the media by alkali-solution or by the color-change dye (i.e. bromcresol purple) previously added to the media.<br> In the present paper, we described a new method using an agar-plate in the sugar fermentation test. An agar-plate containing one sugar as the sole carbon source, yeast extract, peptone, inorganic salts and CaCO3 as the acid indicator was prepared in a 90mm diameter petridish. The agarplate was covered with sterile 1.5% agar. Test strains of lactic acid bacteria were inoculated on the agar-plate by stabbing.<br> The sugar fermentation reaction was easily detected by observing the incubated agar-plates. In positive reaction, a clear zone of dissolved CaCO3 by the acid produced was observed around the stab line on the agar-plate; whereas in negative reaction, the clear zone was not observed. A maximum of 20 lactic acid bacteria strains can be tested on an agar-plate prepared in a 90mm petridish.

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