Simulation of Sucrose Hydrolysis by Yeast.

DOI Web Site Web Site
  • Nagamune Teruyuki
    Chemical Engineering Laboratory, The Institute of Physical and Chemical Research
  • Nakamura Takashi
    Ajinomoto Co., Ltd.
  • Endo Isao
    Chemical Engineering Laboratory, The Institute of Physical and Chemical Research
  • Inoue Ichiro
    Chemical Engineering Laboratory, The Institute of Physical and Chemical Research

Bibliographic Information

Other Title
  • 酵母菌によるスクロース加水分解過程のシミュレーション
  • コウボキン ニ ヨル スクロース カスイ ブンカイ カテイ ノ シミュレーショ

Search this article

Description

The kinetics of the enzymatic hydrolysis of sucrose by cell-bound invertase of yeast (Saccharomyces cerevisiae) and the change in its maximal reaction rate during cultivation were examined in both batch culture and discontinuous-feeding fed-batch culture systems using a synthetic medium whose composition of sugars is almost the same as that in cane sugar molasses (sucrose : glucose : fructose= 2 : 1 : 1). The experimental results showed that the reaction of sucrose inversion by cell-bound invertase of yeast was inhibited not only by substrate sucrose but also by products, competitively by fructose and partially noncompetitively by glucose. It was also observed that the maximal reaction rate of cell-bound invertase was limited at high total sugar concentration and increased as its concentration was lowered. This response of maximal reaction rate against total sugar concentration was expressed in terms of a dimensionless characteristic curve. These results were incorporated into a kinetic model that has proven satisfactory in simulating hydrolysis of sucrose by yeast in batch culture and discontinuous-feeding fed-batch culture systems.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top