酵母菌によるスクロース加水分解過程のシミュレーション

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タイトル別名
  • Simulation of Sucrose Hydrolysis by Yeast.
  • コウボキン ニ ヨル スクロース カスイ ブンカイ カテイ ノ シミュレーショ

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説明

The kinetics of the enzymatic hydrolysis of sucrose by cell-bound invertase of yeast (Saccharomyces cerevisiae) and the change in its maximal reaction rate during cultivation were examined in both batch culture and discontinuous-feeding fed-batch culture systems using a synthetic medium whose composition of sugars is almost the same as that in cane sugar molasses (sucrose : glucose : fructose= 2 : 1 : 1). The experimental results showed that the reaction of sucrose inversion by cell-bound invertase of yeast was inhibited not only by substrate sucrose but also by products, competitively by fructose and partially noncompetitively by glucose. It was also observed that the maximal reaction rate of cell-bound invertase was limited at high total sugar concentration and increased as its concentration was lowered. This response of maximal reaction rate against total sugar concentration was expressed in terms of a dimensionless characteristic curve. These results were incorporated into a kinetic model that has proven satisfactory in simulating hydrolysis of sucrose by yeast in batch culture and discontinuous-feeding fed-batch culture systems.

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