書誌事項
- タイトル別名
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- Control of Gene Expression from the SUC2 Promoter of Saccharomyces cerevisiae using Two Carbon Sources.
- 2シュルイ ノ タンソゲン オ モチイル コウボ SUC2 プロモーター カラ
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抄録
We cultured an oversecretion inutant of Saccharomyces cerevisiae strain which harbors a recombinant plasmid pNA3 containing mouse α-amylase gene fused to SUC2 promoter and signal sequence of 28kDa killer toxin and PGK terminetor. We found that gene expression from the promoter was efficiently derepressed in a medium containing lactic acid as a carbon source. When glucose and lactic acid coexisted in the medium, recombinant yeast grew, using glucose exclusively, and the enzyme was produced using lactic acid after complete depletion of glucose. To control lactic acid concentration at 10 kg/m3, we applied an on-line glucose and lactic acid monitoring and controlling system for jar fermentor culture of this recombinant yeast. By a combination of glucose and lactic acid as carbon sources, effective gene expression from SUC2 promoter could be achieved and 2.23 kat/m3 of a-amylase was produced.
収録刊行物
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- 化学工学論文集
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化学工学論文集 18 (5), 693-700, 1992
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390282679486171136
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- NII論文ID
- 130000863274
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- NII書誌ID
- AN00037234
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- ISSN
- 13499203
- 0386216X
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- NDL書誌ID
- 3784529
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可