Purification Process for .BETA.-Galactosidase Using Aqueous Two-Phase Partitioning and PEG Fractional Precipitation.

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  • KUBOI RYOICHI
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University
  • YAMAHARA KATSUHITO
    Department of Chemical Science and Engineering, Graduate School of Engineering Science, Osaka University

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  • 水性二相分配法とＰＥＧ分画沈殿法を組み合わせたβ‐ｇａｌａｃｔｏｓｉｄａｓｅの分離精製プロセス
  • スイセイ 2ソウ ブンパイホウ ト PEG ブンカク チンデンホウ オ クミア

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Combining aqueous two-phase partitioning and PEG fractional precipitation, a simple and effective purification process for the target intra-cellular enzyme β-galactosidase (β-gal) from E. coli (W 3110) cells was developed based on the evaluated differences between surface properties of β-gal and those of other total soluble proteins. β-gal was partitioned to the PEG rich top phase of the used aqueous two -phase system and finally recovered at high selectivity and yield with the following PEG fractional precipitation mediated by the addition of salt to the separated top phase.

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