ヘミセルラーゼに関する研究(第3報)

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タイトル別名
  • Studies on the Hemicellulases Part III
  • ヘ ミセル ラーゼ ニ カンスル ケンキュウ 3 Aspergillus niger ノ マンナナーゼ ノ セイセイ ト ソノ セイシツ
  • Purification and Some Properties of Mannanase from <i>Aspergillus niger</i> van Tieghem sp
  • <i>Aspergillus niger</i>のマンナナーゼの精製とその性質

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抄録

A mannanase was purified from extracts of bran Koji culture of Aspergillus niger van Tieghem sp. by column chromatography of DEAE-Sephadex and Sephadex G-75, electric focusing method, and zone electrophoresis.<br> The pH optimum of purified mannanase was 3.5, the pH stable range was 4_??_7, and the thermostable range was below 50°C.<br> When the mannanase reacted to galactomannan from soy bean hull, galactomannan from guar gum, mannan from coffee bean and glucomannan from arum root, mannooligosaccharides were found and the hydrolytic limit to those substrates were 12, 12, 58, 39% respectively.<br> So this enzyme was considered as to be endo-β-mannanase.

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