Estimation of methanogenesis by quantification of coenzyme F430 in marine sediments

書誌事項

公開日
2016
資源種別
journal article
DOI
  • 10.2343/geochemj.2.0410
公開者
一般社団法人日本地球化学会

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説明

<p>Coenzyme F430 is the hydrocorphinoid nickel complex which acts as active site in methyl-coenzyme M reductase (MCR). The MCR-F430 complex catalyzes the last step of methanogenesis: reduction of methyl-coenzyme M to methane. Since F430 is a common enzyme for mathanogens, it can be a function-specific biomarker to estimate biomass and activities of methanogens in environmental samples. A recently developed high sensitive method for coenzyme F430 analysis and a further purification step revealed F430 concentrations at 69 and 88 meters below seafloor (mbsf) off Shimokita Peninsula (529 and 31.3 fmol g-wet–1) and at 60 and 275 mbsf at Nankai Trough (31.4 and 26 fmol g-wet–1). Estimated methanogenic biomass and activities based on reported F430 content and activities in methanogens and prokaryotic cell weight, were ranging from 3.9 × 106 to 2.7 × 106 cells g-wet–1 and from 1.7 to 11 μmol d–1 g-wet–1.</p>

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