The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in vitro
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- Equilibrina Ilma
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Krayukhina Elena
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Inoue Kota
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Ishikawa Yuya
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Kawamoto Akihiro
- Graduate School of Frontier Bioscience, Osaka University
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- Suetake Isao
- Institute for Protein Research, Graduate School of Science, Osaka University
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- Takata Hideaki
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Uchiyama Susumu
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Fukui Kiichi
- Department of Biotechnology, Graduate School of Engineering, Osaka University
書誌事項
- タイトル別名
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- The role of phosphorylation of histone H3 at serine 10 in chromatin condensation in <i>vitro</i>
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説明
Post-translational modifications on histone tails play essential roles in modulating chromatin higher order structure. Phosphorylation of histone H3 at serine 10 (H3S10ph) is associated with chromosome condensation. However direct evidence and the molecular mechanism underlying chromosome condensation induced by H3S10ph in human is unclear. Herein, we employed in vitro reconstituted nucleosomal arrays mimicking H3S10ph by substitution S10 to aspartic acid (H3S10D) using human recombinant histones and highly tandem repeats widom 601 sequence. Using analytical ultracentrifugation sedimentation velocity (AUC-SV) analysis and transmission electron microscopy (TEM) observation, we found that there is no significant difference in the local folding and condensation between WT H3 and H3S10D containing nucleosomal arrays. Thus, our results suggest that H3 phosphorylation at serine 10 does not directly play a role in chromatin condensation and thus higher order structure.
収録刊行物
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- Chromosome Science
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Chromosome Science 18 (1-2), 9-14, 2015
財団法人 染色体学会
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詳細情報 詳細情報について
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- CRID
- 1390282679545675264
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- NII論文ID
- 130005114061
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- NII書誌ID
- AA11163057
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- ISSN
- 21850852
- 13441051
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- NDL書誌ID
- 027010231
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可