書誌事項
- タイトル別名
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- High Sensitivity Detection of ATP Using Bioluminescence at An Optical Fiber End
- ヒカリ ファイバー センタン デノ セイブツ ハッコウ オ リヨウシタ コウカンド ATP ケンシュツ
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説明
We investigated the sensitivity of ATP detection based on bioluminescence at an optical fiber end where luciferase molecules were immobilized via silica-binding protein molecules. Luminescence was detected by an avalanche photo diode (APD), with coupling optics to make full use of the merit of compactness, high quantum efficiency and low noise of the APD. The core diameter and the numerical aperture of the optical fiber, as well as the design of the coupling optics, were optimized so as to realize high photon-collection efficiency. A detection limit of about 10-10 M was obtained, which corresponds to 10-15 mol of ATP. A rough estimation shows that the photon count rate is still two orders of magnitude lower than that limited by diffusion or reaction processes, implying a possibility of further improvement of the sensitivity.
収録刊行物
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- 電気学会論文誌C(電子・情報・システム部門誌)
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電気学会論文誌C(電子・情報・システム部門誌) 127 (10), 1498-1503, 2007
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390282679583167232
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- NII論文ID
- 10019958118
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- NII書誌ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL書誌ID
- 8960116
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可