cAMP-Independent Chloride Secretion Activated by a Vasoactive Intestinal Peptide in a Monolayer Culture of Human Bronchial Epithelial Cells.

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書誌事項

公開日
1999
資源種別
journal article
DOI
  • 10.1248/bpb.22.745
公開者
公益社団法人 日本薬学会

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説明

To investigate the effects of vasoactive intestinal peptide (VIP) on Cl- transport across normal human bronchial epithelial (NHBE) cells grown in a monolayer, changes in short-circuit current (Isc) were measured in Ussing chamber systems. In the presence of 10-4M amiloride, the addition of VIP to the serosal solution led to an increase in the Isc in a concentration-dependent manner, the 50% effective concentration (EC50) being 2.6×10-11M. However, the addition of 10-5M forskolin had little effect on the increase in Isc. On the other hand, in the intracellular cAMP measurement, 10-5M forskolin remarkably increased the cAMP levels, but 10-7M VIP did not.This result suggests that Cl- secretion by VIP is not related to the raised intracellular cAMP levels in NHBE cells.

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