Significant Expression of Functional Human Type 1 Mitochondrial ADP/ATP Carrier in Yeast Mitochondria.
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- HATANAKA Takashi
- Faculty of Pharmaceutical Sciences, University of Tokushima
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- TAKEMOTO Yukiyo
- Faculty of Pharmaceutical Sciences, University of Tokushima
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- HASHIMOTO Mitsuru
- Faculty of Pharmaceutical Sciences, University of Tokushima
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- MAJIMA Eiji
- Faculty of Pharmaceutical Sciences, University of Tokushima APRO Life Science Institute
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- SHINOHARA Yasuo
- Faculty of Pharmaceutical Sciences, University of Tokushima
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- TERADA Hiroshi
- Faculty of Pharmaceutical Sciences, University of Tokushima
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抄録
As a first step to characterize the unknown functional properties of the human mitochondrial ADP/ATP carrier (AAC), we tried to express human type 1 AAC (hAAC1) in Saccharomyces cerevisiae. Expression of hAAC1 in yeast mitochondrial membrane was very low, although its transcript level was high. Its expression was improved greatly by replacement of its N-terminal region with the corresponding region of yeast type 2 AAC (yAAC2), as observed with the bovine type 1 AAC (bAAC1). This chimeric hAAC1 showed similar ADP transport activity to that of chimeric bAAC1, corresponding to the transport activity of bAAC1 in bovine heart mitochondria. These results suggested that the N-terminal region of yAAC2 is important for expression of the mammalian carriers in yeast mitochondria. Using the present expression system, studies on the functional properties of the human AAC isoforms in relation to their structures are now possible.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (6), 595-599, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679603177088
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- NII論文ID
- 110003638522
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXktFygsbg%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5793233
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- PubMed
- 11411543
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可