cDNA Cloning of the Sry-Related Gene Sox6 from Rat with Tissue-Specific Expression.
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- Narahara Masanori
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Yamada Akihisa
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Hamada-Kanazawa Michiko
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Kawai Yuichi
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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- Miyake Masaharu
- Faculty of Pharmaceutical Sciences, Kobe-Gakuin University
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Abstract
A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this cDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologues. The expression of Sox6 was determined in rat by Northern hybridization and Real-time quantitative reverse transcription (RT)-PCR. rSox6 (rat Sox6) was specifically expressed in the neonatal brain and adult testis with Northern blotting. Real-time quantitative RT-PCR for the determination of Sox6 mRNA was examined. The rSox6 was expressed in the neonatal brain and adult testis as well as by Northern blotting and also expressed in the adult eyeball and slightly in the ovary.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 25 (6), 705-709, 2002
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679604566656
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- NII Article ID
- 110003638743
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XkvV2hu7g%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 6174897
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- PubMed
- 12081133
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed