ウシ歯根膜におけるProstaglandin E1の影響 Succinate‐1.4‐`14´C由来の有機酸代謝及び新生高分子について
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- 目黒 千代恵
- 神奈川歯科大学口腔生化学教室
書誌事項
- タイトル別名
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- Effect of prostaglandin E<SUB>1</SUB> on bovine periodontal ligament
- Succinate-1, 4-<SUP>14</SUP>C由来の有機酸代謝及び新生高分子について
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抄録
Prostaglandins have been recognized recently as one of the important chemical mediators in periodontal disease. The present study is aimed at studying the metabolic alteration in periodontal tissues mediated by prostaglandins.<BR>The effect of prostaglandin E1 (5 to 200ng/ml) on bovine periodontal ligament cells was investigated by using the short term incubation system with succinate-1, 4-14C as the radioactive tracer. The extracellular medium was analysed for TCA cycle intermediates and synthesized macromolecules.<BR>Organic acid analysis showed that the synthetic ratios of the pyruvate: malate, and citrate:α ketoglutarate were altered by PGE1. Especially, α-ketoglutarate: isocitrate ratio increased markedly with 5ng PGE1/ml.<BR>Macromolecular sythesis was inhibited at low concentration and accelerated at high concentration of PGE1. The products over 65 K dalton decreased about 1/3 with 5ng PGE1/ml, and increased with 200ng PGE1/ml, but the substances of 32-65 K dalton increased about 1/4 with 5ng PGE1/ml and decreased 15.6% with 200ng PGE1/ml.<BR>It was found that the synthesized macromolecules of 59 K dalton estimated on the slab SDS polyacrylamide gel electrophoresis was remarkably induced by the low concentration of PGE1 (5ng/ml).<BR>These results demonstrated that PGE1 is one of the mediators of periodontal tissue cells alterated their organic acid metabolism and macromolecular synthesis.
収録刊行物
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- 歯科基礎医学会雑誌
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歯科基礎医学会雑誌 26 (2), 341-357, 1984
歯科基礎医学会
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詳細情報 詳細情報について
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- CRID
- 1390282679658649856
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- NII論文ID
- 130003815056
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- ISSN
- 03850137
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可