A case of aspergillosis. Application of double staining with fluorescence dye for fungi.

DOI 8 References
  • FURUTA Tomoko
    Second Department of Pathology, Kinki University School of Medicine
  • KIMURA Masatomo
    Second Department of Pathology, Kinki University School of Medicine
  • OTSUKA Kayo
    Second Department of Pathology, Kinki University School of Medicine
  • UESUGI Tadao
    Section of Hospital Pathology, Kinki University School of Medicine
  • CHIKUGO Takaaki
    First Department of Pathology, Kinki University School of Medicine
  • YAGI Yuji
    Department of Neurology, Kinki University School of Medicine
  • SATO Takao
    Second Department of Pathology, Kinki University School of Medicine
  • HASHIMOTO Shigeo
    Second Department of Pathology, Kinki University School of Medicine

Bibliographic Information

Other Title
  • アスペルギルス症の１例　真菌用蛍光染色液を用いた重染色の試み
  • Application of double staining with fluorescencedye for fungi
  • 真菌用蛍光染色液を用いた重染色の試み

Search this article

Description

We report an autopsy case of Aspergillus brain abscess that had extended from Aspergillus sinusitis, and attempted to detect the fungus in cytology specimens byFungiflora Y® and Papanicolaou double staining. An 80-year-old man was found to have right orbital and sphenoidal aspergillosis. Treatment with antifungal agents was ineffective, and a brain abscess was detected by magnetic resonance imaging seven months later. The patient's condition deteriorated and he died. Pus from the brain abscess was used to makes smear and Fungiflora Y®-Papanicolaou double staining was performed. It was difficult to detect the fungi on screening with the ×10 objective lens of light microscope because of their faint color. However, under a fluorescence microscope the fungal hyphae were apple green and it was very easy to detect them. Thus, Fungiflora Y®-Papanicolaou double staining seems very useful for detecting fungal hyphae on smear cytology.

Journal

References(8)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top