Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated from Physarum polycephalum: Signaling Events of Antiproliferative Action by PHYLPA.
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- Murakami-Murofushi Kimiko
- Department of Biology, Faculty of Science, Ochanomizu University
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- Kaji Kazuhiko
- Tokyo Metropolitan Institute of Gerontology
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- Fukuda Mitsugu
- Tokyo Metropolitan Institute of Gerontology
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- Murofushi Hiromu
- Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo
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- Kano Kazutaka
- department of Physiological Chemistry and Nutrition, Faculty of Medicine
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- Shioda Masaki
- department of Physiological Chemistry and Nutrition, Faculty of Medicine
書誌事項
- 公開日
- 1993
- 資源種別
- journal article
- DOI
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- 10.1247/csf.18.363
- 公開者
- 日本細胞生物学会
この論文をさがす
説明
The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol (16), inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-phases proceeded to G2- and G1-phases, respectively, and most of cells were arrested at G1- or G2- phase during PHYLPA treatment. The growth was recovered when PHYLPA was removed from the medium. In the presence of serum, PHYLPA did not show obvious inhibitory effects, indicating the existence of a factor(s) which neutralizes the antiproliferative activity of PHYLPA.<br> PHYLPA elicited an increase in 3', 5' -cyclic adenosine monophosphate (cAMP) in a biphasic fashion in fibroblast cells. It also elicited inositol phosphate accumulation, as well as a transient rise in cytoplasmic free Ca2+ ion.
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 18 (5), 363-370, 1993
日本細胞生物学会
