Establishment and Characterization of Human Peripheral Nerve Microvascular Endothelial Cell Lines : A New in vitro Blood-Nerve Barrier (BNB) Model
-
- Abe Masaaki
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Sano Yasuteru
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Maeda Toshihiko
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Shimizu Fumitaka
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Kashiwamura Yoko
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Haruki Hiroyo
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Saito Kazuyuki
- Department of Neurology and Neurological Science, Tokyo Medical and Dental University Graduate School Department of Neurology and Neurological Science, Tokyo Medical and Dental University Graduate School
-
- Tasaki Ayako
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Kawai Motoharu
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
-
- Terasaki Tetsuya
- Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
-
- Kanda Takashi
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
書誌事項
- タイトル別名
-
- Establishment and Characterization of Human Peripheral Nerve Microvascular Endothelial Cell Lines: A New <i>in vitro</i> Blood-Nerve Barrier (BNB) Model
この論文をさがす
抄録
The blood-nerve barrier (BNB) is a highly specialized unit that maintains the microenvironments of the peripheral nervous system. Since the breakdown of the BNB has been considered a key step in autoimmune neuropathies such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyraduculoneuropathy, it is important to understand the cellular properties of the peripheral nerve microvascular endothelial cells (PnMECs) which constitute the BNB. For this purpose, we established an immortalized cell line derived from human PnMECs. The human PnMECs were transduced with retroviral vectors encoding the temperature-sensitive SV40 large T antigen and human telomerase. This cell line, termed FH-BNB, showed a spindle fiber-shaped morphology, expression of von Willebrand factor and uptake of acetylated low density lipoprotein. These cells expressed tight junction proteins including occludin, claudin-5, ZO-1 and ZO-2 at the cell-cell boundaries. P-glycoprotein and GLUT-1 were also detected by a Western blot analysis and the cells exhibited the functional expression of p-glycoprotein. In addition, transendothelial electrical resistance experiments and paracellular permeabilities of sodium fluorescein and fluorescein isothiocyanate-labeled dextran of molecular weight 4 kDa across these cells demonstrated that FH-BNBs had functional tight junctions. These results indicated that FH-BNBs had highly specialized barrier properties and they might therefore be a useful tool to analyze the pathophysiology of various neuropathies.
収録刊行物
-
- Cell Structure and Function
-
Cell Structure and Function 37 (2), 89-100, 2012
日本細胞生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679672516480
-
- NII論文ID
- 130004137580
- 40019581994
-
- NII書誌ID
- AA0060007X
-
- COI
- 1:STN:280:DC%2BC38npsV2jsg%3D%3D
-
- ISSN
- 13473700
- 03867196
-
- NDL書誌ID
- 024272253
-
- PubMed
- 22672995
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可