A Rapid and Simple Method Using the Detergent Triton X114 for Preparation of Outer Membrane Proteins from Bacteria Containing Mycolic Acids.

  • Aizawa Tomoko
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Iwabuchi Noriyuki
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Kikuchi Aki
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Urai Makoto
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Anzai Hirosi
    Laboratory of Applied Biochemistry, Department of Agriculture, Junior College, Nihon University
  • Sunairi Michio
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Nakajima Mutsuyasu
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University

書誌事項

タイトル別名
  • rapid and simple method using the detergent Triton X114 for preparation of outer membrane proteins from bacteria containing mycolic acids

この論文をさがす

説明

A rapid and simple method using the detergent Triton X114 to prepare outer membrane proteins from whole cells of bacteria containing mycolic acids, α-alkyl, β-hydroxyl fatty acids is described. SDS-PAGE analysis and subsequent N-terminal amino acid sequencing revealed that the proteins prepared from whole cells by this method were almost identical to outer membrane proteins prepared by the conventional cell-disruption and differential-centrifugation method. Therefore, this method is useful to prepare outer membrane proteins from whole cells of Mycolata, bacteria containing mycolic acids.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (58)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ