Assay of Urinary Enzyme in a Dog

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  • 犬の尿中酵素活性測定について
  • イヌ ノ ニョウチュウ コウソ カッセイ ソクテイ ニ ツイテ

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In a dog, gentamicin (10mg/kg) was given every 8 hours for 9 days to produce experimental renal failure. Then urinalysis showed an increase in urine volume, a decrease in urinary specific gravirty, proteinuria, glucosuria, celluria and enzymuria. Elevation in the activities of the urinary enzymes preceded a rise in levels of creatinine (Cr) and BUN in the serum. Activities of serum Cr rose by day 10 and of BUN by day 14. Urinary LDH, γ-GTP, NAG and ALP began to rise steadily by day 7 and increased rapidly after that. Urinary LAP rose slightly by day 12 and rapidly after that. Urinary LDH5 increased with a rapid rise of total urinary LDH activity. Renal biopsy specimens on days 5 and 9 were examined histologically, showing granular degeneration of the proximal tubules. Necropsy was made on day 16. The renal lesions were characterized by hydropic degeneration and necrotic changes in the proximal tubules and occasional interstitial infiltration of macrophages. Accordingly, it was suggested that assays of urinary enzymes might be more sensitive indicators of renal damage than conventional renal function tests in the small animal practice.

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