書誌事項
- タイトル別名
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- Preparation of Abalone Enzymes Capable of Cell Wall Degradation of a Marine Red Alga Porphyra yezoensis (Bangiales, Rhodophyta)
- アワビ ショウカカン ユライ ノ カイサン コウソウ スサビノリ サイボウヘキ ブンカイ コウソ ノ チョウセイ
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We extracted a xylanase and a mannanase from Ezo abalone Haliotis discus hannai, and examined the enzyme activity and the ability for cell wall degradation of Porphyra yezoensis. Abalone xylanase was partially purified from the crude enzyme prepared from the digestive fluid by column chromatography on a TOYOPEARL CM-650M. Abalone mannanase was successfully purified from the crude enzyme by column chromatographies on TOYOPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW-50E The gametophytic thalli of P.yezoensis could not be degraded by the xylanase alone. The purified mannanase could degrade the thalli into cell masses consisting of 10-20 cells; however, it produced practically no protoplasts from the thalli. When the thalli were incubated both with the xylanase and the purified mannanase, some protoplasts could be produced from the thalli. The two enzymes may be useful for the studies on cell engineering and asexual seedling production of this alga.
収録刊行物
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- 水産増殖
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水産増殖 54 (1), 83-88, 2006
日本水産増殖学会
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詳細情報 詳細情報について
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- CRID
- 1390282679692824448
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- NII論文ID
- 10017970434
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- NII書誌ID
- AN00124667
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- ISSN
- 21850194
- 03714217
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- NDL書誌ID
- 7889929
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可