GISH法によるタバコ種間雑種(Nicotiana gossei Domin×N. tabacum L., N. rustica L., ×N. tabacum L.)での親由来染色体の識別

DOI Web Site Web Site 被引用文献4件 参考文献14件

書誌事項

タイトル別名
  • Identification of Parental Chromosomes in the Interspecific Hybrids of Nicotiana rustica L.*N. tabacum L. and N. gossei Domin*N. tabacum L., Using Genomic in situ Hybridization.
  • Identification of parental chromosomes in the interspecific hybrids of Nicotiana rustica L.×N. tabacum L.and N.gossei Domin×N.tabacum L.,using genomic in situ hybridization
  • Identification of parental chromosomes

この論文をさがす

抄録

Genomic in situ hybridization was carried out for mitotic cells of the interspecific hybrids of Nicotiana rustica L. (2n = 48)× N. tabacum L. (2n = 48) and N. gossei Domin (2n = 36)× N. tabacum L. Without blocking DNA of N. tabacum, clear hybridization signals from the biotinylated total genomic DNA of N. rustica or N. gossei, were obtained for the set of chromosomes, indicating that each parent could be discriminated. In the hybrid of N. rustica × N. tabacum, twenty-four chromosomes among forty-eight chromosomes were confirmed to be derived from N. rustica. Furthermore, two chromosomes exhibited regions with weak hybridization signals, suggesting that interspecific translocations might have occurred between N. rustica and N. tabacum chromosomes. On the other hand, in the hybrid of N. gossei× N. tabacum, eighteen chromosomes showing a hybridization signal over the entire length, were discriminated from the forty-two hybrid chromosomes. No region with weak signal was detected in any of them.

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (14)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ