Development of plants resistant to <i>Papaya leaf distortion mosaic virus</i> by intergeneric hybridization between <i>Carica papaya</i> and <i>Vasconcellea cundinamarcensis</i>

DOI Web Site Web Site 1 Citations 29 References Open Access
  • Tarora Kazuhiko
    Okinawa Prefectural Agricultural Research Center Department of Bioscience and Textile Technology, Shinshu University
  • Shudo Ayano
    Okinawa Prefectural Agricultural Research Center
  • Kawano Shinji
    Department of Agriculture, Forestry and Fisheries, Yaeyama Agriculture, Forestry and Fisheries Promotion Center
  • Yasuda Keiji
    Okinawa Prefectural Forest Resources Research Center
  • Ueno Hiroki
    Department of Bioscience and Textile Technology, Shinshu University Institute of Vegetable and Floriculture Science, NARO
  • Matsumura Hideo
    Gene Research Center, Shinshu University
  • Urasaki Naoya
    Okinawa Prefectural Agricultural Research Center

Bibliographic Information

Other Title
  • Development of plants resistant to Papaya leaf distortion mosaic virus by intergeneric hybridization between Carica papaya and Vasconcellea cundinamarcensis

Search this article

Description

<p>In this study, we confirmed that Vasconcellea cundinamarcensis resists Papaya leaf distortion mosaic virus (PLDMV), and used it to produce intergeneric hybrids with Carica papaya. From the cross between C. papaya and V. cundinamarcensis, we obtained 147 seeds with embryos. Though C. papaya is a monoembryonic plant, multiple embryos were observed in all 147 seeds. We produced 218 plants from 28 seeds by means of embryo-rescue culture. All plants had pubescence on their petioles and stems characteristic of V. cundinamarcensis. Flow cytometry and PCR of 28 plants confirmed they were intergeneric hybrids. To evaluate virus resistance, mechanical inoculation of PLDMV was carried out. The test showed that 41 of 134 intergeneric hybrid plants showed no symptoms and were resistant. The remaining 93 hybrids showed necrotic lesions on the younger leaves than the inoculated leaves. In most of the 93 hybrids, the necrotic lesions enclosed the virus and prevented further spread. These results suggest that the intergeneric hybrids will be valuable material for PLDMV-resistant papaya breeding.</p>

Journal

  • Breeding Science

    Breeding Science 66 (5), 734-741, 2016

    Japanese Society of Breeding

Citations (1)*help

See more

References(29)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top