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The effect of chemicals on somatic homologous recombination in the rice blast fungus: its possible application for detection of mycotoxins
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- ARAZOE Takayuki
- Graduate School of Agriculture, Meiji University
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- OHSATO Shuichi
- Graduate School of Agriculture, Meiji University
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- MAEDA Kazuyuki
- Graduate School of Agriculture, Meiji University Present address: Graduate School of Bioagricultural Sciences, Nagoya University
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- ARIE Tsutomu
- Graduate School of Agriculture, Tokyo University of Agriculture and Technology
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- KUWATA Shigeru
- Graduate School of Agriculture, Meiji University
Bibliographic Information
- Other Title
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- イネいもち病菌の体細胞相同組換えに及ぼす化学薬剤の影響：マイコトキシン検出系としての可能性について
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Description
We previously established a detection/selection system for somatic homologous recombination (HR), which is one of the genetic modification mechanisms in eukaryotes. Because HR is stimulated by the protein synthesis inhibitor blasticidin S, it is presumed that HR in Pyricularia oryzae can be induced by various chemical stresses. To evaluate the effects of chemical stresses on the frequency of HR, several chemical agents were applied to P. oryzae and HR were detected using our detection system. Three well-known DNA-damaging agents－methyl methanesulfonate, bleocin, and methyl viologen－considerably increased the frequency of HR. Adding the amino acid synthesis inhibitor bialaphos, or the protein synthesis inhibitor T-2 toxin, to the medium also significantly increased the frequency of HR. These results suggest that the increased frequency of HR caused by inhibitors of the primary metabolic pathway reflect destabilization of the genome by chemical stressors. Taken together, these findings suggest that the HR detection system may become one of the most useful biological assays for detecting mycotoxins.
Journal
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- JSM Mycotoxins
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JSM Mycotoxins 64 (2), 141-146, 2014
Japanese Society of Mycotoxicology
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Details 詳細情報について
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- CRID
- 1390282679764517120
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- NII Article ID
- 130004699346
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- NII Book ID
- AA12721071
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- ISSN
- 18810128
- 02851466
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- NDL BIB ID
- 027209181
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- Text Lang
- ja
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed
