IMMUNOCYTOCHEMICAL STUDIES OF GLUTAMATE-LIKE IMMUNOREACTIVE NEURONS IN THE RAT HYPOTHALAMUS

DOI

Bibliographic Information

Other Title
  • ラット視床下部におけるグルタミン酸含有ニューロンの免疫電顕的観察
  • —Synaptic Interactions between Glutamate and Somatostatin Neurons—
  • ―ソマトスタチン含有ニューロンとの形態学的相関について―

Abstract

Ultrastructure of glutamate-like immunoreactive (Glu-LI) neurons in the rat hypoth-alamus was studied immunocytochemically using Glu antibody. Synaptic Interactions between Glu-LI neurons and somatostatin-like immunoreactive (SRIF-LI) neurons were examined ultrastru-cturally by a pre-embedding double immunostaining technique combining the silver-gold intensified peroxidase-antiperoxidase complex-3, 3'-diaminobenzidine (SGI-PAP-DAB) method with the nonintensified ABC-DAB method. Glu-LI axon terminals were found directly terminating on the basal lamina of the blood capillaries of the portal vessels in the median eminence. Glu-LI neurons received synaptic inputs from unknown neurons in the arcuate nucleus. Synaptic interactions between Glu-LI and SRIF-LI neurons were found in the periventricular nucleus. In the arcuate nucleus, synapses occurred between Glu-LI axons and dendrites. These findings suggest that Glu-LI neurons may regulate the secretory activity of growth hormone-producing cells directly or indirectly through synapses with SRIF-LI neurons.

Journal

Details

  • CRID
    1390282679811134336
  • NII Article ID
    130001824659
  • DOI
    10.14930/jsma1939.57.56
  • ISSN
    21850976
    00374342
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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