Analysis of Dynamics of Orally Administered Antigens in Peyer's Patches and Interaction between Antigen-loaded Dendritic Cells and Antigen-Specific T Cells

DOI
  • KOMAGATA Yoshinori
    Graduate School of Medicine, University of Tokyo Department of Internal Medicine, Teikyo University School of Medicine
  • NAGATANI Katsuya
    Graduate School of Medicine, University of Tokyo Division of Rheumatology and Clinical Immunology, Jichi Medical University
  • YAMAMOTO Kazuhiko
    Graduate School of Medicine, University of Tokyo

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Other Title
  • 経口投与された抗原のパイエル板における動態および樹状細胞と抗原特異的T細胞との相互作用の解析
  • ―パイエル板における抑制性T細胞生成の可能性―
  • —Potential for Induction of Regulatory T Cells in Peyer's Patches—

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Although it has been shown that orally administered antigens can induce systemic immune tolerance (i.e., oral tolerance), the underlying detailed mechanism has not yet been elucidated. In this study, we aimed to analyze the dynamics of oral antigens and cellular interactions in Peyer's patches (PPs). Fluorescence-labeled ovalbumin (OVA) was orally administered to BALB/c mice and was found to be captured by dendritic cells (DCs) in the SED region of PPs. When mice were first transferred with OVA-specific naïve T cells derived from OVA-TCR transgenic mice and then orally administered OVA, these mice showed accumulation of T cells in the IFR region of PPs. The accumulated T cells were then collected and analyzed for the pattern of gene expression by real-time PCR, which revealed a gene expression pattern similar to that of FoxP3-positive regulatory T (Treg) cells. CCR9, an intestinal homing marker, was also strongly expressed. These results suggest that DCs that have captured oral antigens in PPs locally induce antigen-specific naïve T cells to differentiate into Treg cells with the intestinal homing property.

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