Lung toxicity assessment using bronchoalveolar lavage fluid and pleural lavage fluid cytology by intratracheal treatment in rats
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- Takehara Hiroshi
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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- Makita Maki
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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- Tanaka Ryota
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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- Tsuchiya Mai
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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- Naya Masato
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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- Hayashi Makoto
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
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Abstract
Usefulness of bronchoalveolar lavage fluid (BALF) and pleural cavity lavage fluid (PLF) as an experimental material was evaluated for the assessment of pulmonary toxicity of chemicals in rats. From the viewpoint of safety, isoflurane can be used for euthanasia/anesthesia because there was no difference in biological properties of BALF between diethyl ether and isoflurane. Here, we also recognized phosphate buffered saline (PBS) and distilled water equally as a solvent/vehicle for negative control. PLF is also provided as a useful target material as well as BALF for assessing chemical lung toxicity. To evaluate the method, we used zinc chloride as a model chemical and obtained the expected and satisfied results. We may conclude that the intratracheal treatment and combination usage of BALF and PLF as a target material is a good method for assessment of chemical pulmonary (lung and plural cavity) toxicity in rats.
Journal
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- The Journal of Toxicological Sciences
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The Journal of Toxicological Sciences 39 (1), 141-145, 2014
The Japanese Society of Toxicology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679880818176
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- NII Article ID
- 130004447106
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- NII Book ID
- AN00002808
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- COI
- 1:STN:280:DC%2BC2czkvFSlsQ%3D%3D
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- ISSN
- 18803989
- 03881350
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- NDL BIB ID
- 025244185
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- PubMed
- 24418718
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed