Lung toxicity assessment using bronchoalveolar lavage fluid and pleural lavage fluid cytology by intratracheal treatment in rats
-
- Takehara Hiroshi
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
-
- Makita Maki
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
-
- Tanaka Ryota
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
-
- Tsuchiya Mai
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
-
- Naya Masato
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
-
- Hayashi Makoto
- Public Interest Incorporated Foundation BioSafety Research Center (BSRC)
この論文をさがす
抄録
Usefulness of bronchoalveolar lavage fluid (BALF) and pleural cavity lavage fluid (PLF) as an experimental material was evaluated for the assessment of pulmonary toxicity of chemicals in rats. From the viewpoint of safety, isoflurane can be used for euthanasia/anesthesia because there was no difference in biological properties of BALF between diethyl ether and isoflurane. Here, we also recognized phosphate buffered saline (PBS) and distilled water equally as a solvent/vehicle for negative control. PLF is also provided as a useful target material as well as BALF for assessing chemical lung toxicity. To evaluate the method, we used zinc chloride as a model chemical and obtained the expected and satisfied results. We may conclude that the intratracheal treatment and combination usage of BALF and PLF as a target material is a good method for assessment of chemical pulmonary (lung and plural cavity) toxicity in rats.
収録刊行物
-
- The Journal of Toxicological Sciences
-
The Journal of Toxicological Sciences 39 (1), 141-145, 2014
一般社団法人 日本毒性学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282679880818176
-
- NII論文ID
- 130004447106
-
- NII書誌ID
- AN00002808
-
- COI
- 1:STN:280:DC%2BC2czkvFSlsQ%3D%3D
-
- ISSN
- 18803989
- 03881350
-
- NDL書誌ID
- 025244185
-
- PubMed
- 24418718
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可