Genotoxicity and reactive oxygen species production induced by magnetite nanoparticles in mammalian cells

DOI Web Site Web Site PubMed 被引用文献4件 参考文献45件 オープンアクセス
  • Kawanishi Masanobu
    Graduate School of Science and Radiation Research Center, Osaka Prefecture University
  • Ogo Sayaka
    Graduate School of Science and Radiation Research Center, Osaka Prefecture University
  • Ikemoto Miho
    Graduate School of Science and Radiation Research Center, Osaka Prefecture University
  • Totsuka Yukari
    Division of Cancer Development System, National Cancer Center Research Institute
  • Ishino Kousuke
    Division of Cancer Development System, National Cancer Center Research Institute
  • Wakabayashi Keiji
    Graduate School of Nutritional and Environmental Sciences, University of Shizuoka
  • Yagi Takashi
    Graduate School of Science and Radiation Research Center, Osaka Prefecture University Department of Life Science, Dongguk University Seoul

書誌事項

公開日
2013
資源種別
journal article
DOI
  • 10.2131/jts.38.503
公開者
一般社団法人　日本毒性学会

この論文をさがす

説明

We examined the genotoxicity of magnetite nanoparticles (primary particle size: 10 nm) on human A549 and Chinese hamster ovary (CHO) AA8 cells. Six hours’ treatment with the particles dose-dependently increased the frequency of micronuclei (MN) in the A549 and CHO AA8 cells up to 5.2% and 5.0% at a dose of 200 µg/ml (34 µg/cm2), respectively. In A549 cells, treatment with the nano­particles (2 µg/ml) for 1 hr induced H2AX phosphorylation, which is suggestive of DNA double strand breaks (DSB). Treating CHO AA8 cells with 2 µg/ml (0.34 µg/cm2) magnetite for 1 hour resulted in a five times higher frequency of sister chromatid exchange (SCE) than the control level. We detected reactive oxygen species (ROS) in CHO cells treated with the particles. These findings indicate that magnetite nano­particles induce ROS in mammalian cells, leading to the direct or indirect induction of DSB, followed by clastogenic events including MN and SCE.

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (45)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ