Dynamic analysis of secretagogue-induced amylase secretion from rat pancreatic acini studied by perifusion system.
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- IMAMURA Kouichiro
- The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University
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- WAKASUGI Hideyuki
- The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University
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- SHINOZAKI Hiroshi
- The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University
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- IBAYASHI Hiroshi
- The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University
Bibliographic Information
- Other Title
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- Dynamic Analysis of Secretagogue induce
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Abstract
A perifusion system was applied for the study on stimulus-enzyme secretion coupling in dispersed pancreatic acini. The system is highly simple, preserves the acini up to more than 3hr, and makes feasible clear-cut examination on the time course of enzyme secretion caused by secretagogues.<br>Caerulein (10-9M) and carbamylcholine (10-5M) caused a biphasic amylase secretory pattern consisting of an initial burst secretion and a sustained one. Caerulein induced a persistent amylase release even after cessation of the stimulation, while carbamylcholine-stimulated amylase release returned to basal levels. Atropine inhibited completely carbamylcholine-stimulated amylase release and the successive stimulation by caerulein evoked the amylase secretion with a decreased initial burst secretion. In calcium free medium, caerulein and carbamylcholine induced only a slight secretion, particularly in the sustained secretion phase and a gradual increase occurred with the addition of calcium.
Journal
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 33 (5), 687-698, 1983
THE PHYSIOLOGICAL SOCIETY OF JAPAN
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Details 詳細情報について
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- CRID
- 1390282680019669760
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- NII Article ID
- 130003404212
- 40005307879
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- NII Book ID
- AA00691224
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- COI
- 1:CAS:528:DyaL2cXjtVansg%3D%3D
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- ISSN
- 18811396
- 0021521X
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- NDL BIB ID
- 2620821
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- PubMed
- 6199537
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed