A Rapid Method for Serodiagnosis of Japanese Encephalitis using Persistently Infected C6/J-121 Cells

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  • KIMURA Tomoaki
    Virsu Laboratory, Osaka Prefectural Institute of Public Health
  • OTAKE Toru
    Virsu Laboratory, Osaka Prefectural Institute of Public Health
  • UEBA Noboru
    Virsu Laboratory, Osaka Prefectural Institute of Public Health
  • MINEKAWA Yoshiichi
    Virsu Laboratory, Osaka Prefectural Institute of Public Health

Bibliographic Information

Other Title
  • 日本脳炎ウイルス持続感染C6/36細胞を用いた血清学的迅速診断法について
  • ニホン ノウエン ウイルス ジゾク カンセン C6 36 サイボウ オ モチイ

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Description

The usefullness of persistently infected C6/36 (C6/J-121) cells with Japanese encephalitis virus (JEV) for a rapid serodiagnostic test was examined with the sera of men, swine and laboratory animals by indirect immunofluorescent antibody (IFA) technique.<BR>Detection of specific antibodies was completed within 3 to 5 hours.<BR>Nonspesific fluorescence frequently observed in other IFA tests was few.<BR>The sensitivity of antibody detection and the serological specificity of IFA (IgG) were similar to those of hemagglutination-inhibition (HI) test.<BR>For the detection of IgM antibodies in sera of JE-patients and naturally JEV infected swine, this method was found to be simpler and more sensitive and rapid than the conventional HI test which required 2-ME treatment of the sera.

Journal

  • Kansenshogaku Zasshi

    Kansenshogaku Zasshi 64 (3), 264-268, 1990

    The Japanese Association for Infectious Diseases

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