Quantitative Detection of<I>Vibrio vulnificus</I>in Seafood
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- KUDO-HARA Yukiko
- Division of Microbiology, National Institute of Health Sciences
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- MIWA Norinaga
- Shizuoka Institute of Environment and Hygiene
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- YAMASAKI Syougo
- Nagasaki Prefectural Institute of Public Health and Environmental Science
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- YATSUYANAGI Jun
- Akita Prefectural Institute of Public Health
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- IWADE Yoshito
- Mie Prefectural Science and Technology
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- TAKAHASHI Hajime
- Division of Microbiology, National Institute of Health Sciences
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- MIYASAKA Jiro
- Kumamoto Prefectural Institute of Public Health and Environmental Science
Bibliographic Information
- Other Title
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- 魚介類からの<I>Vibrio vulnificus</I>の定量検出方法の検討
- 魚介類からのVibrio vulnificusの定量検出方法の検討
- ギョカイルイ カラ ノ Vibrio vulnificus ノ テイリョウ ケンシュツ ホウホウ ノ ケントウ
- Quantitative Detection ofVibrio vulnificusin Seafood
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Abstract
To quantify the number ofVibrio vulnificusin shellfish, we compared the most probable number (MPN) combined with a culture (MPN-culture) or polymerase-chain reaction (PCR) assay (MPNPCR) to a quantitative PCR assay. Enrichment in alkaline peptone water by MPN was conducted at 25 and 35°C. Enrichment at 35°Cwas superior or similar to enrichment at 25°Cinover 65% of samples by MPNculture and in more than 75% of samples by MPN-PCR assay.V. vulnificuswas more easily isolated on chromogenic agar medium during culture, MPN-PCR assay was superior or similar to MPNculture in over 90% of samples by enrichment at 25°C and to over 88% of samples by enrichment at 35°C. The number ofV. vulnificusby quantitative PCR assay was similar to that of MPN-PCR assay in 6 of 8 samples but not from MPNculture. V. vulnificus contamination was frequently detected in samples from Kyushu Island.
Journal
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- Kansenshogaku Zasshi
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Kansenshogaku Zasshi 79 (12), 931-936, 2005
The Japanese Association for Infectious Diseases
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Keywords
Details 詳細情報について
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- CRID
- 1390282680025165184
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- NII Article ID
- 130004331237
- 10018629372
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- NII Book ID
- AN00047715
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- ISSN
- 1884569X
- 03875911
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- NDL BIB ID
- 7781554
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed