3. 凍結乾燥によるマウス精子の保存(セミナー「低温と乾燥による生物保存技術への挑戦～マミーエンジニアリング」)

DOI 機関リポジトリ Web Site 参考文献36件
  • 鈴木 宏志
    帯広畜産大学原虫病研究センターゲノム機能学分野:東京大学大学院医学系研究科発生・医療工学講座
  • 川瀬 洋介
    中外医科学研究所

書誌事項

タイトル別名
  • 3. Freeze-drying Spermatozoa in Mice(Papers presented at the Seminar, "The latest developments in biopreservation technigues using low temperature and low moisture conditions", July 23, 2007, Tokyo)
  • 凍結乾燥によるマウス精子の保存
  • トウケツ カンソウ ニ ヨル マウス セイシ ノ ホゾン

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Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried spermatozoa were reported in the cow and rabbit prior to 1960. Unfortunately, these preliminary results have not been confirmed. Spermatozoa become defective in motility after freeze-drying and are unable to fertilize eggs both in vivo and in vitro. Since freeze-drying of mammalian spermatozoa was demonstrated without loss of genetic or reproductive potential, recent investigations of freeze-dried sperm have focused on the factors affecting freeze-drying spermatozoa in an effort to store the male gamete at ambient temperature. It is particularly essential to assure long-term preservation for several decades or centuries. Recent findings indicate that the pressure levels at primary drying of freeze-dried spermatozoa, in addition to the components of the suspending solution, appears to be an important factor for long-term preservation of freeze-dried spermatozoa in mice.

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