Double Transgenic Mice Crossed GFP-LC3 Transgenic Mice With .ALPHA.MyHC-mCherry-LC3 Transgenic Mice Are a New and Useful Tool to Examine the Role of Autophagy in the Heart
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- Terada Mai
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Nobori Kiyoshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Munehisa Yoshiko
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Kakizaki Manabu
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Ohba Takayoshi
- Department of Physiology, Akita University Graduate School of Medicine
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- Takahashi Yoichiro
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Koyama Takashi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Terata Yutaka
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Ishida Masaru
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Iino Kenji
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Kosaka Toshimitsu
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Watanabe Hiroyuki
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Hasegawa Hitoshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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- Ito Hiroshi
- Department of Cardiovascular Medicine, Akita University Graduate School of Medicine
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説明
Background: The involvement of autophagy in heart disease has been reported. Transgenic mice expressing GFP-LC3 have been a useful tool in detecting autophagosomes systemically. It is difficult to differentiate increased formation of autophagosomes from decreased clearance of autophagosomes in the heart using GFP-LC3 mice. Methods and Results: We generated transgenic mice expressing mCherry-LC3 under αMyHC promoter and crossed the mice with transgenic mice expressing GFP-LC3. The deference of resistance to acidic conditions between GFP and mCherry overcame the limitation. Conclusions: This method is an innovative approach to examine the role of autophagy and to analyze autophagosome maturation in cardiomyocytes. (Circ J 2010; 74: 203 - 206)<br>
収録刊行物
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- Circulation Journal
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Circulation Journal 74 (1), 203-206, 2010
一般社団法人 日本循環器学会
