The Action of Two α-Amylases, TVA I and TVA II, from The rmoactinomyces vulgaris R-47 on Glucosyl Cyclodextrin
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- SHIMURA Yoichiro
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- TONOZUKA Takashi
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- SAKANO Yoshiyuki
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
Bibliographic Information
- Other Title
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- 好熱性放線菌Theymoactinornyces vulgaris R-47からクローン化された二つのα-アミラーゼ,TVAIとTVAII,のグルコシルサイクロデキストリン分解に関する作用
Abstract
We performed a kinetic study to estimate the action mode of two α-amylases, TVA I and TVA II, on glucosyl cyclodextrins. The enzyme genes from Thermoactinomyces vulgaris R-47 were individually cloned and expressed in Escherichia coli, and the enzymes were prepared from the transformants. TVA I hydrolyzed glucosyl-γ-cyclodextrin well, whereas TVA II hydrolyzed glucosyl-α- and -β-cyclodextrins. TVA I and TVA II hydrolyzed glucosyl cyclodextrins to produce glucose, maltose, panose, and 62-α-glucosylmaltotriose.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 45 (4), 393-395, 1998
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282680147092224
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- NII Article ID
- 130004091142
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- COI
- 1:CAS:528:DyaK1MXos1ynug%3D%3D
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- ISSN
- 18844898
- 13403494
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed