好熱性放線菌Theymoactinornyces vulgaris R-47からクローン化された二つのα-アミラーゼ,TVAIとTVAII,のグルコシルサイクロデキストリン分解に関する作用
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- 志村 洋一郎
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- 殿塚 隆史
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- 坂野 好幸
- Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology
書誌事項
- タイトル別名
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- The Action of Two α-Amylases, TVA I and TVA II, from The rmoactinomyces vulgaris R-47 on Glucosyl Cyclodextrin
説明
We performed a kinetic study to estimate the action mode of two α-amylases, TVA I and TVA II, on glucosyl cyclodextrins. The enzyme genes from Thermoactinomyces vulgaris R-47 were individually cloned and expressed in Escherichia coli, and the enzymes were prepared from the transformants. TVA I hydrolyzed glucosyl-γ-cyclodextrin well, whereas TVA II hydrolyzed glucosyl-α- and -β-cyclodextrins. TVA I and TVA II hydrolyzed glucosyl cyclodextrins to produce glucose, maltose, panose, and 62-α-glucosylmaltotriose.
収録刊行物
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- 応用糖質科学
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応用糖質科学 45 (4), 393-395, 1998
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282680147092224
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- NII論文ID
- 130004091142
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- COI
- 1:CAS:528:DyaK1MXos1ynug%3D%3D
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- ISSN
- 18844898
- 13403494
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可