Production and Application of Maltose Phosphorylase and Trehalose Phosphorylase by a Strain of Plesiomonas

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Other Title
  • プレシオモナスによるマルトースホスホリラーゼおよびトレハロースホスホリラーゼの生産と応用

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Description

A strain of Plesiomonas capable of producing intracellular thermostable maltose phosphorylase (MP) and trehalose phosphorylase (TP), which were useful for the production of trehalose from maltose, was isolated from mud of a Japanese seashore. The isolate (SH-35) grew well among the ranges of pH and temperature at pH 6-9 and 15-45°C with optima at pH 7.0 and 37°C by shaking cultivation, though the maximum yield of these enzymes were obtained at pH 7.5 and 34°C for MP and pH 8.0 and 37°C for TP with medium containing maltose as a carbon source and the mixture of Polypepton-S, yeast extract and urea as nitrogen sources. Optimum pH and temperature for producing trehalose were pH 7-8 and 55-60°C in the presence of 10-40% (w/w) maltose and 5-50 mM inorganic phosphate, and about 60% (as dry basis) of maltose was converted into trehalose by the simultaneous action of both enzymes before and after extraction from cells under the best conditions. These microbial and enzymatic characteristics are consistent with the industrial production of trehalose from maltose.

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Details 詳細情報について

  • CRID
    1390282680148765824
  • NII Article ID
    130004257437
  • DOI
    10.11541/jag1994.42.19
  • COI
    1:CAS:528:DyaK2MXltlyitLc%3D
  • ISSN
    18844898
    13403494
  • Data Source
    • JaLC
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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