Caveolae-Independent Activation of Protein Kinase A in Rat Neonatal Myocytes
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- Fukutomi Masashi
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
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- Nishida Motohiro
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
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- Maruyama Yoshiko
- Laboratory of Cellular Signaling, Graduate School of Pharmaceutical Sciences, University of Tokyo
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- Kobayashi Hiroyuki
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
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- Kurose Hitoshi
- Department of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, Kyushu University
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抄録
Cardiomyocytes express both β1- and β2-adrenergic receptors, and these receptors play a differential role in chronotropic and inotropic effects of the heart. Caveolae are known as an important regulator of G-protein-coupled receptor signaling. In the present report, we examined whether caveolae have a role in β-adrenergic receptor-stimulated cAMP production and protein kinase A activation in neonatal myocytes. Isoproterenol-stimulated cAMP production was mediated by β1- and β2-subtypes, which depends on the receptor number of each subtype. However, protein kinase A activation was exclusively mediated by the β1-subtype. Disruption of caveolae by methyl-β-cyclodextrin treatment did not affect the relative contribution of subtypes to isoproterenol-stimulated cAMP production. β1-Subtype-mediated protein kinase A activation was also not affected by the disruption of caveolae. These results suggest that β1-adrenergic receptor-mediated protein kinase A activation is compartmentalized and independent of caveolae.<br>
収録刊行物
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 98 (2), 168-174, 2005
公益社団法人 日本薬理学会
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詳細情報 詳細情報について
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- CRID
- 1390282680152452096
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- NII論文ID
- 10025728483
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- NII書誌ID
- AA11806667
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- ISSN
- 13478648
- 13478613
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- NDL書誌ID
- 7337896
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- PubMed
- 15942126
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可