書誌事項
- タイトル別名
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- Estimation of Avian Prolactin by Polyacrylamide Disc Electrophoresis
- ディスク デンキ エイドウホウ ニヨル ニワトリ プロラクチン カッセイ ノ
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抄録
The possibility for the estimation of prolactin in the fowl pituitary by a disc-electrophoresis was investigated. Homogenates of anterior pituitaries from broiler chickens were subjected to the disc-electrophoresis using polyacrylamide gel and divided into 5 segments according to their mobilities. For comparison, NIH-prolactin (ovine) was treated in the same way. Eluates of each segment were assayed for prolactin using a local pigeon crop-gland response. 1. The fastest migrating band was found to be the band which includes prolactin. 2. In the ovine prolactin, a close relationship was found between the dose of eluate and the optical density, and a high correlation coefficient was obtained between the optical density and the crop-gland response. 3. Similar results were also obtained in the eluates of the fastest migrating band in the disc-electrophoresis of the chicken pituitary homogenates. The results indicate that the method using the disc-electrophoresis can be used as a sensitive and simplified method for the quantitative analysis of the fowl prolactin.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 48 (3), 168-172, 1977
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390282680168655360
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- NII論文ID
- 130000733889
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 1817591
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可